Effects of TF-TP on Human Retinal Pigment Epithelial Cell Damage Induced by Blue Light

Effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells.

AIM To evaluate the effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial (RPE) cells. METHODS Three concentrations (0.06 mg/ml, 0.6 mg/ml, and 4 mg/ml) of trypan blue were applied to human ARPE19 cells for 1 minute. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RPE cells were sampled ...

Simulated microgravity induced damage in human retinal pigment epithelial cells.

PURPOSE The goal of this study was to determine the potential damage to the human retina that may occur from weightlessness during space flight using simulated microgravity. METHODS Human retinal pigment epithelial (hRPE) cells were cultured for 24 h in a National Aeronautics and Space Administration-designed rotating wall bioreactor vessel to mimic the microgravity environment of space. Sing...

Effects of bioflavonoids on cultured human retinal pigment epithelial cells

Effects of light-emitting diode radiations on human retinal pigment epithelial cells in vitro.

Human visual system is exposed to high levels of natural and artificial lights of different spectra and intensities along lifetime. Light-emitting diodes (LEDs) are the basic lighting components in screens of PCs, phones and TV sets; hence it is so important to know the implications of LED radiations on the human visual system. The aim of this study was to investigate the effect of LEDs radiati...

Blueberry anthocyanins: protection against ageing and light-induced damage in retinal pigment epithelial cells.

Retinal pigment epithelium (RPE) cells are vital for retinal health. However, they are susceptible to injury with ageing and exposure to excessive light, including UV (100-380 nm) and visible (380-760 nm) radiation. To evaluate the protective effect of blueberry anthocyanins on RPE cells, in vitro cell models of replicative senescent and light-induced damage were established in the present stud...